For Total Viable Count Medium Feature The medium consists of non-selective medium and redox indicator of 2,3,5-Triphenyl Tetrazolium Chloride (TTC).Most colonies grown on TC are Red color. Interpretation Incubate 48 hours at 35 ± 2℃. Count all colonies grown on the plate.
For Coliform and Escherichia coli Feature Medium contains two kinds of chromogenic enzyme substrate, Magenta-GAL and X-GLUC.Red/Pink colonies for other coliform than E.coli while Blue/Blue Purple color for E. coli are observed respectively Interpretation Incubate 24 hours at 35 ± 2℃.For E. coli, count all Blue/Blue Purple colonies. Combinedtotal number of both colonies of…
For Vibrio Paraheaemolyticus Feature Colonies grown on VP plate are distinguished by blue/blue-green color for Vparaheamolyticus and by red/pink color for V.cholerea, V.vulnifius and by milk-white color for other Vibri spp.as Valginolyticus. Interpretation Incubate 18-20 hours at 35 ± 2 ºC. Count all Blue/Blue Green colred colonied as V.parahaemolyticus.
For Yeast and Mold Feature Medium contains chromogenic enzyme substrate X-Phos that develops blue color for most of yeast (the other yeast does not generate Blue color), and antibiotics that inhibit the growth of bacteria. Mold forms the cottony colonies with characteristic color. Interpretation Incubate 3-7 days at 20-25℃.Count Blue or White to Cream…
For Staphylococcus aureus Feature Staphylococcus aureus generates Light blue/Blue colonies. Some bacteria other than Staphylococcus aureus may grow and generate white and/or red colonies Interpretation Incubate for 24 ± 2 hours at 35 ± 2 ºC.
For Salmonella Detection Feature Salmonella in the specimen is distinguished by thecombination of different three test principles,alkalizaion of the medium by Salmonella’s lysinedecarboxylase ability (medium color will change Blue-purple to Yellow), changed colony color to Green by chromogenic substrate (Black colonies are generated by hydrogen sulfide producing Salmonella) and motility of Salmonella. Operating Procedure
For Bacillus cereus Interpretation Bacillus cereus generates green/blue colonies. Incubate for 24 ± 2 hours at 35 ± 2 ºC.