PRImeDETECT™ Salmonella Plus PCR Detection Kit
PRImeDETECT™ Salmonella Plus Detection Kit (Salmonella spp & Listeria monocytogenes) is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR method (multiplex PCR).
The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
- Cat. No. FP0010 – 48 reactions
- Cat. No. FP0011 – 96 reactions
Specifications
| Target | Simultaneously detect and differentiate Salmonella spp. and Listeria monocytogenes in the same tube |
| Inclusivity | Salmonella: Positive in 30 reference strains of food origin from the Spanish Type Culture Collection (CECT). Listeria: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections. |
| Exclusivity | Salmonella: Tested with 29 nontarget strains. Listeria: 100% Tested with 94 nontarget strains composed of 51 strains of non-Listeria monocytogenes and 43 strains of non-Listeria. |
| Detection | probe labelled with fluorescent dyes– Salmonella: HEX-BHQ1; Listeria: FAM-BHQ1a; IAC:CY5-BHQ2. |
| Thermal Cyclers | Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers |
| Shipment | Ambient Temperature |
| Storage Conditions | -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. |
| Shelf Life | 12 months from the date of purchase, if it is properly stored |
Advantages
- Easy and Ready-to-use Kit.
- Fast: results in less than 24 hours with minimal handling.
- Reliable and highly specific Results.
- High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility