PRImeDETECT™ Legionella spp PCR Detection Kit
An ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method.
The Kit includes all reagents required in a comfortable ready-to-use PCR MasterMix. In addition, the kit includes both Positive Control and Negative Control. The Positive Control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
- Cat. No. FP0030 – 48 reactions
- Cat. No. FP0031 – 96 reactions
Specifications
| Target | Legionella spp. |
| Inclusivity | 100%. Primers and Probes have been tested satisfactorily in 29 strains of Legionella. |
| Exclusivity | 100%. Primers and Probes have been tested satisfactorily in 28 non-Legionella bacteria |
| Detection | Probe labelled with fluorescent dyes– Legionella: FAM-TAMRA; IAC: JOE-TAMRA |
| Thermal Cyclers | Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers. |
| Shipment | -20 ºC using gel packs |
| Storage Conditions | -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. |
| Shelf Life | 6 months from the date of purchase, if it is properly stored |
Advantages
- Easy and Ready-to-use Kit.
- Fast: results in less than 24 hours with minimal handling.
- Reliable and highly specific Results.
- High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility.