PRImeDETECT™ STEC Detection Kit is an ideal tool for an specific, rapid, and reliable Amplification and Detection of the virulence-associated genes stx1, stx2 and eae by the real-time PCR method (Multiplex PCR).
The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
- Cat. No. FP0035 – 48 reactions
- Cat. No. FP0036 – 96 reactions
|Target||Shiga toxin-producing E. coli|
|Inclusivity||specifically reacts with all strains of E. coli with the indicated target genes (stx1, stx2 and eae).|
|Exclusivity||No cross-reactions with other strains of Non-Shiga toxin-producing E. coli (STEC)|
|Detection||probe labelled with fluorescent dyes– FAM-BHQ1a (sxt1), HEX-BHQ1(stx2), ROX-BHQ2 (eae) and CY5-BHQ1(IAC).|
|Thermal Cyclers||Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers|
|Storage Conditions||-20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.|
|Shelf Life||12 months from the date of purchase, if it is properly stored|
- Easy and Ready-to-use Kit.
- Fast: results in less than 24 hours with minimal handling.
- Reliable and highly specific Results.
- High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility