Escherichia coli is a coliform which is found in the gut of warm-blooded animals making it an excellent indicator for fecal contamination. Not all E. coli are pathogenic but there are strains which can be considered as opportunistic bacteria infecting an immunocompromised host. Testing for the presence of coliform and E. coli is important in food industry, especially in monitoring water quality.
- Medium contains two kinds of chromogenic enzyme substrate, Magenta-GAL and X-GLUC, then red/pink color colonies for Coliform and blue/blue purple color colonies for E. coli are observed respectively.
- AOAC International: 35±2°C for 24±2 hours
- MicroVal and NordVal: 37±1°C for 24±2 hours
- E. coli – Blue colonies
- Total coliform – Red, blue, and purple colonies