Staphylococcus aureus is a common human microflora, it can naturally be found on the skin, nose, and hair. Due to this, it is commonly used as index of food handlers’ personal hygiene. Thus, high staphylococcal population in food system indicates poor hygiene and sanitation. They are normally harmless but at high population, can begin forming endotoxins causing “staph” food poisoning. They can also tolerate low water activity and are opportunistic in high sugar food.
There is no need of egg yolk suspension by the combination of selective agents and chromogenic substrates.
Blue/blue green colonies for Staphylococcus aureus are observed, and fishing of colonies is easy.
- AOAC International: 35±2℃ for 24±2 hours
- MicroVal and NordVal: 37±1℃ for 24±2 hours
- Count Light blue/blue colonies for S. aureus