Megazyme K-YBGL: β-glucan Assay Kit Yeast and Mushroom
Product code: K-YBGL
β-Glucan (Yeast and Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom preparations.
New, improved procedure.
Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.
Reference:
McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Linear Range: | 4 to 100 μg of glucose per assay |
| Limit of Detection: | 1 g/100 g |
| Total Assay Time: | ~ 100 min |
| Application examples: | Yeast preparations, mushroom preparations and other materials. |
| Method recognition: | Novel method |