Megazyme E-AMGDF: Amyloglucosidase (Aspergillus niger)
Product code: E-AMGDF
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
| Enzyme Activity: | Amyloglucosidase |
| EC Number: | 3.2.1.3 |
| CAZy Family: | GH15 |
| CAS Number: | 9032-08-0 |
| Synonyms: | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase |
| Source: | Aspergillus niger |
| Molecular Weight: | 143,500 |
| Expression: | From Aspergillus niger |
| Specificity: | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. |
| Specific Activity: | ~ 3,260 U/mL (40oC, pH 4.5 on soluble starch) ~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside) |
| Unit Definition: | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC. |
| Temperature Optima: | 70oC |
| pH Optima: | 4 |
| Application examples: | Applications for the measurement of starch and dietary fiber in the food and feeds industry. |
| Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
