Product code: E-AMGDF
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
|Synonyms:||glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase|
|Expression:||From Aspergillus niger|
|Specificity:||Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.|
|Specific Activity:||~ 3,260 U/mL (40oC, pH 4.5 on soluble starch)
~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside)
|Unit Definition:||One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC.|
|Application examples:||Applications for the measurement of starch and dietary fiber in the food and feeds industry.|
|Method recognition:||AOAC Method 2000.11 and GB Standard 5009.245-2016|