Megazyme K-FRUC: Fructan Assay Kit
Product code: K-FRUC
The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).
New, improved procedure.
In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.
The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Fructan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 410 |
| Signal Response: | Increase |
| Linear Range: | 2.3 to 55 µg of D-fructose or D-glucose per assay |
| Limit of Detection: | 0.16 g/100 g |
| Total Assay Time: | ~ 90 min |
| Application examples: | Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials |
| Method recognition: | AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III |