Description

The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies.

Features & Benefits

• High Fidelity – >90x wild type Taq
• Extreme Speed – up to 3x faster PCR results with extension rates as fast as 1 kb/sec (for fragments less than 1 kb in size)
• Tough Tested – tolerant to a wide range of PCR inhibitors
• Superior Sensitivity – higher yields from lower inputs as little as 2 pg
• Long Range – amplify +24 kb gDNA, +40 kb λDNA

Contents

2x reaction buffer containing optimized concentrations of MgCl2, dNTP’s and proprietarily formulated HiFi polymerase, hot start antibodies and ToughMix chemistry