Harlequin® Listeria Chromogenic Agar (Ottaviani & Agosti) (LCA)
Item No. NCM1004
A selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs as described in ISO 11290-1:2017.
LCA Agar forms part of Neogen’s innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR.
Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium’s selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli.
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies.
Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).
This medium is not intended to diagnose diseases or other conditions in humans.
| Specifications | |||||||||||||||||||||||||||
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| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media, One Broth One Plate | ||||||||||||||||||||||||||
| Result Type | Presence/Absence | ||||||||||||||||||||||||||
| Approvals | This product forms part of Neogen’s innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR. | ||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 70.50 | ||||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||||
| Plates per 500 g | 390.00 | ||||||||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see the appropriate supplement data sheet for availability and preparation instructions. |
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| Previous Item Number | Product was previously known as LabM Product: HAL010 Harlequin® Listeria Chromogenic Agar | ||||||||||||||||||||||||||
| Storage Conditions | 2-8 °C away from direct sunlight | ||||||||||||||||||||||||||
| Package Weight | 0.31 lb | ||||||||||||||||||||||||||
