Megazyme E-BSPRT: Protease (Subtilisin A from Bacillus licheniformis)
Product code: E-BSPRT
High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Dietary Fiber test method.
| Enzyme Activity: | Protease |
| EC Number: | 3.4.21.62 |
| CAS Number: | 9014-01-1 |
| Synonyms: | subtilisin; subtilisin A |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 30,250 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
| Specific Activity: | ~ 6 U/mg of protein (40oC, pH 8.0 on casein) |
| Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
