Megazyme E-BLAAM: α-Amylase (Bacillus licheniformis)
Product code: E-BLAAM
High purity α-Amylase (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Dietary Fiber methods, suitable for use at pH 6.5 and above.
| Enzyme Activity: | α-Amylase |
| EC Number: | 3.2.1.1 |
| CAZy Family: | GH13 |
| CAS Number: | 9000-90-2, 9000-85-5 |
| Synonyms: | alpha-amylase; 4-alpha-D-glucan glucanohydrolase |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 58,000 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | endo-hydrolysis of α-1,4-D-glucosidic linkages in starch. |
| Specific Activity: | ~ 55 U/mg (40oC, pH 6.5 on Ceralpha reagent) |
| Unit Definition: | One Unit of α-amylase is the amount of enzyme required to release one µmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.0 and 40oC. |
| Temperature Optima: | 75oC |
| pH Optima: | 6.5 |
| Application examples: | For use in Megazyme Dietary Fiber methods. |
| Method recognition: | EBC Method 6.5 |
