| Enzyme Activity: | β-Galactosidase |
| EC Number: | 3.2.1.23 |
| CAZy Family: | GH35 |
| CAS Number: | 9031-11-2 |
| Synonyms: | beta-galactosidase; beta-D-galactoside galactohydrolase |
| Source: | Aspergillus niger |
| Molecular Weight: | 125,000 |
| Concentration: | Supplied at ~ 4,000 U/mL |
| Expression: | Purified from Aspergillus niger |
| Specificity: | Hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. |
| Specific Activity: | ~ 240 U/mg (40oC, pH 4.5 on p-nitrophenyl β-D-galactoside) |
| Unit Definition: | One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC. |
| Temperature Optima: | 60oC |
| pH Optima: | 5 |
| Application examples: | Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries. |
| Content: | 8,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
