Megazyme E-ISAMY: Isoamylase (Glycogen 6-glucanohydrolase)
Product code: E-ISAMY
Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
| Enzyme Activity: | Isoamylase |
| EC Number: | 3.2.1.68 |
| CAZy Family: | GH13 |
| CAS Number: | 9067-73-6 |
| Synonyms: | isoamylase; glycogen 6-alpha-D-glucanohydrolase |
| Source: | Pseudomonas sp. |
| Molecular Weight: | 71,500 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Purified from Pseudomonas sp. |
| Specificity: | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
| Specific Activity: | ~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg) |
| Unit Definition: | One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC. |
| Temperature Optima: | 50oC |
| pH Optima: | 4 |
| Application examples: | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
| Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
