Megazyme K-RHAMNOSE: L-Rhamnose Assay Kit
Product code: K-RHAMNOSE
The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
L-Rhamnose occurs naturally in the L-form and is commonly present as a component of the carbohydrate moiety of eukaryotic glycoproteins and in plant cell wall polysaccharides. The most abundant occurrence of L-rhamnose is within the pectic fraction of plant cell wall polysaccharides. L-Rhamnose is commonly used as a non-metabolisable marker along with lactulose for dual-permeability testing in the diagnosis of intestinal diseases such as Crohn’s disease or coeliac disease.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
| Content: | 50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Rhamnose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 5 to 100 µg of L-rhamnose per assay |
| Limit of Detection: | ~ 1.2 mg/L |
| Reaction Time (min): | ~ 5 min at 25oC or ~ 4 min at 37oC |
| Application examples: | Hydrolysates of plant material and polysaccharides, culture media / supernatants and other materials. |
| Method recognition: | Novel method |
