Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. AccuStart Genotyping ToughMix enables probe-based genetic analysis (SNP detection and allelic discrimination) directly from crude extracts, DBS punches, plant tissue and clinical specimens.
Features & Benefits
- Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for critical, single-nucleotide polymorphism (SNP) detection assays.
- Easy-to-use 2x concentrated SuperMix with AccuVue plate loading dye and pre-blended passive reference dye simplifies reaction setup
Single tube, 2X concentrated reagent containing: Reaction buffer (MgCl2, dATP, dCTP, dGTP, dTTP), AccuStart II Taq DNA Polymerase, Inert AccuVue dye, Proprietary enzyme stabilizers and performance-enhancing additives, and Titrated reference dye (if applicable).
|Genotyping ToughMix, ROX||1250 x 20 µl rxns (10 x 1.25 ml)||95116-012|
|5000 x 20 µl rxns (1 x 50 ml)||95116-05K|
|Genotyping ToughMix, Low ROX||1250 x 20 µl rxns (10 x 1.25 ml)||95117-012|
|5000 x 20 µl rxns (1 x 50 ml)||95117-05K|