Megazyme K-AVCHO: Available Carbohydrates Assay Kit
Product code: K-AVCHO
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose.
New Improved method receiving ‘First Action’ status: AOAC 2020.07.
This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.
|Content:||100 assays of each per kit|
|Storage Temperature:||Short term stability: 2-8oC,
Long term stability: See individual component labels
|Stability:||> 2 years under recommended storage conditions|
|Analyte:||Available Carbohydrates, Dietary Fiber|
|Linear Range:||4 to 80 μg of D-glucose, D-fructose or D-galactose per assay|
|Limit of Detection:||1.475 g/100 g|
|Reaction Time (min):||~ 5 h|
|Application examples:||Food ingredients, food products and other materials.|
|Method recognition:||AOAC Method 2020.07|